Journal: Oncology Reports

Article Title: NR4A1 mediates chemotherapy-induced senescence via the PI3K/AKT pathway in gastric cancer cells

doi: 10.3892/or.2026.9080

Figure Lengend Snippet: OXA induces senescence was verified in gastric cancer cells. (A and B) SA-β-Gal staining of Ctrl and OXA groups and quantification of SA-β-Gal positive cells in AGS and MKN45 cells. (C) p21 protein expression and quantification. (D and E) Immunofluorescence of γ-H2AX and quantification. Red fluorescence indicates γ-H2AX staining, and blue fluorescence reflects nuclear staining with DAPI. (F) mRNA levels of the senescence-associated secretory phenotype factors. (G) CDK4, cyclin D1, RB, p-RB protein expression, and quantification in the two groups. Data represent the mean ± SD of at least three independent experiments. *P<0.05, **P<0.01 and ***P<0.001. OXA, oxaliplatin; SA-β-Gal, senescence-associated β-galactosidase; p-, phosphorylated.

Article Snippet: AGS and MKN45 human GC cell lines were acquired from the Procell Life Science & Technology Co., Ltd. and maintained in RPMI-1640 containing 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) at 37°C with 5% CO 2 condition.

Techniques: Staining, Expressing, Immunofluorescence, Fluorescence

Journal: Nature Communications

Article Title: Multitrait GWAS and functional validation reveal genetic loci for gastric cancer

doi: 10.1038/s41467-026-70774-9

Figure Lengend Snippet: A mRNA levels were measured by RT‑qPCR in AGS and MKN45 cells transfected with control (CON) or gene‑specific overexpression plasmids for NDUFA12 , FGD6 , NR2C1 or VEZT at 48 h. Data are presented as the mean ± SD from n = 3 biologically independent experiments. Significance relative to the CON for each gene was assessed using an unpaired two‑tailed t ‑test (no adjustment for multiple comparisons) and denoted as * P < 0.05, ** P < 0.01 and *** P < 0.001; ns not significant. Exact P values are provided in the figure. B Cell viability was monitored over time. Data are presented as the mean ± SD from n = 3 biologically independent experiments, each performed in triplicate wells. Significance at each time point relative to CON was determined by two‑way ANOVA followed by Tukey’s multiple comparisons test and denoted as ns not significant, * P < 0.05, ** P < 0.01 and *** P < 0.001. Exact P values are provided in the figure. C , D EdU staining ( C ) and colony formation assays ( D ) were performed in transfected AGS and MKN45 cells. Images are representative of n = 3 biologically independent experiments. Scale bar, 100 μm. CON control, OP overexpression.

Article Snippet: The HEK293T, AGS and MKN45 cell lines were obtained from Procell (Wuhan, China) and cultured in DMEM (for HEK293T), Ham’s F-12K (for AGS) or RPMI-1640 (for MKN45) medium (all from Gibco, Carlsbad, CA, USA), each supplemented with 10% foetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Transfection, Control, Over Expression, Staining

Journal: Nature Communications

Article Title: Multitrait GWAS and functional validation reveal genetic loci for gastric cancer

doi: 10.1038/s41467-026-70774-9

Figure Lengend Snippet: Wound-healing ( A ; scale bar, 250 μm) and Transwell migration ( B ; scale bar, 100 μm) assays were performed using AGS and MKN45 cells transfected with control (CON) or gene‑specific overexpression (OP) plasmids. Images are representative of three biologically independent experiments with consistent results. CON control, OP overexpression.

Article Snippet: The HEK293T, AGS and MKN45 cell lines were obtained from Procell (Wuhan, China) and cultured in DMEM (for HEK293T), Ham’s F-12K (for AGS) or RPMI-1640 (for MKN45) medium (all from Gibco, Carlsbad, CA, USA), each supplemented with 10% foetal bovine serum (FBS; Gibco) and 1% penicillin/streptomycin (Gibco).

Techniques: Migration, Transfection, Control, Over Expression